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Using RAPD in genotyping and transmission detection

ZHANG Tiejun, ZHOU Xiaoming, ZHANG Tao, YU Shunzhang, JIANG Qingwu, CHEN Yue

Frontiers of Medicine 2008, Volume 2, Issue 3,   Pages 269-275 doi: 10.1007/s11684-008-0051-9

Abstract: Different RAPD primers sequence was used for amplification and their differentiating capabilities forrelatively better RAPD fingerprint maps, subsequently, using selected RAPD primer could give out a group of amplificationpolymerase chain reaction bands.

Keywords： amplification polymerase     CTAB     sequence     cetyltrimethylammonium     RAPD    

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Strategy for Carbon Sink and Its Amplification in Marine Fisheries

Tang Qisheng and Liu Hui

Strategic Study of CAE 2016, Volume 18, Issue 3,   Pages 68-73 doi: 10.15302/J-SSCAE-2016.03.011

Abstract: management of natural carbon sink and environment of China's seas; implementing fisheries carbon sink amplification

Keywords： marine fisheries carbon sink     carbon sink fisheries     carbon sink amplification     conservation and management    

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Effects of 3-aminobenzamide on poly (ADP-ribose) polymerase expression, apoptosis and cell cycle progression

DU Xiang, ZHAO Hongguang, GUO Wei, GONG Shouliang, WANG Wen

Frontiers of Medicine 2008, Volume 2, Issue 2,   Pages 204-206 doi: 10.1007/s11684-008-0039-5

Abstract: study the changes of apoptosis and cell cycle progression in HeLa cells after the poly (ADP-ribose) polymerase

Keywords： control     irradiation     apoptotic     progression     3-AB    

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Using pyrosequencing and quantitative PCR to analyze microbial communities

Husen ZHANG

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 1,   Pages 21-27 doi: 10.1007/s11783-011-0303-9

Abstract: In parallel, quantitative real-time polymerase chain reaction (QPCR) allows quantitative monitoring of

Keywords： polymerase chain reaction (PCR)     microbial communities     pyrosequencing     gut     microbial fuel cell     sludge    

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Advances in newly developing therapy for chronic hepatitis C virus infection

Paul J. Pockros

Frontiers of Medicine 2014, Volume 8, Issue 2,   Pages 166-174 doi: 10.1007/s11684-014-0334-2

Abstract: In late 2013 the first nucleotide polymerase inhibitor regimen with RBV alone for genotypes 2/3 and in

Keywords： direct-acting antiviral (DAA)     nucleotide polymerase inhibitors     protease inhibitors    

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Abnormal expression of c-Myc in human bronchial epithelial cells malignantly transformed by anti-BPDE

FU Juan, JIANG Yiguo, CHEN Xuemin

Frontiers of Medicine 2008, Volume 2, Issue 4,   Pages 380-385 doi: 10.1007/s11684-008-0073-3

Abstract: vehicle-treated control cells (16HBE-N) by using different methods respectively, including reverse transcriptase-polymerase

Keywords： transcriptase-polymerase     vehicle-treated     understood     molecular mechanism     different    

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Smartphone-Imaged HIV-1 Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) on a Chip Article

Gregory L. Damhorst,Carlos Duarte-Guevara,Weili Chen,Tanmay Ghonge,Brian T. Cunningham,Rashid Bashir

Engineering 2015, Volume 1, Issue 3,   Pages 324-335 doi: 10.15302/J-ENG-2015072

Abstract: In this paper, we perform reverse-transcription loop-mediated isothermal amplification (RT-LAMP) on minimallyOur integrated assay shows amplification from as few as three viruses in a ~ 60 nL RT-LAMP droplet, corresponding

Keywords： human immunodeficiency virus (HIV)     viral load     loop-mediated isothermal amplification     smartphone     point-of-care    

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The study of forced vibration for threetower suspension bridge under moving loads

Feng Zhaoxiang,Chen Liang and Miu Changqing

Strategic Study of CAE 2012, Volume 14, Issue 5,   Pages 80-84

Abstract: However, the load amplification factor of structural force and displacement does not vary with the quantity

Keywords： dynamic characteristics     forced vibration     response     load amplification factor    

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Measurement and Control Technology for the Array of Low Cost PCR Microreactors

Li Xianming ,Zhang Yulin ,Li Jiantian

Strategic Study of CAE 2007, Volume 9, Issue 7,   Pages 76-81

Abstract:

In this paper a new style of polymerase chain reaction(PCR)equipment

Keywords： microreactors     measurement     control     polymerase chain reaction    

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Experimental Study on the Piezoelectric Prestage Driver of Electrohydraulic Servo Valves

CHENG Guang-ming, SHEN Chuan-liang, YANG Zhi-gang

Frontiers of Mechanical Engineering 2006, Volume 1, Issue 1,   Pages 56-59 doi: 10.1007/s11465-005-0017-4

Abstract: multilayer actuator was amplified by a flexure-hinged amplifier based on the principles of triangle amplification

Keywords： flexure-hinged amplifier     kHz     amplification     high-frequency     electromagnetic    

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Expression and clinical implication of PRL-1 and PRL-3 in transitional cell carcinoma of bladder

Bin HAO, Changwei LIU, Huixiang LI

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 197-203 doi: 10.1007/s11684-009-0036-3

Abstract: The expression of PRL-1 and PRL-3 mRNA was detected by using reverse transcription polymerase chain reaction

Keywords： bladder     phosphatase of regenerating liver 1     phosphatase of regenerating liver 3     reverse transcription polymerase    

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High-level expression of recombinant IgG1 by CHO K1 platform

Ningning Xu, Jianfa Ou, Al-Karim (Al) Gilani, Lufang Zhou, Margaret Liu

Frontiers of Chemical Science and Engineering 2015, Volume 9, Issue 3,   Pages 376-380 doi: 10.1007/s11705-015-1531-5

Abstract: Post transfection, the production of cell pool was optimized by glutamine-free selection and amplificationconstruction of high producing cell line, from gene to clone, could be completed within six month and the gene amplification

Keywords： Chinese hamster ovary (CHO)     monoclonal antibody     IgG1     amplification     cell line development    

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Seasonal microbial community shift in a saline sewage treatment plant

Qingmei YAN, Xuxiang ZHANG, Tong ZHANG, Herbert H P FANG

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 1,   Pages 40-47 doi: 10.1007/s11783-011-0304-8

Abstract: community shift along with environmental variations using denaturing gradient gel electrophoresis of polymerase

Keywords： sewage treatment plants     polymerase chain reaction (PCR)     denaturing gradient gel electrophoresis (DGGE    

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Effect of intestinal ischemia/reperfusion injury on leptin and orexin-A levels

LIN Ji, YAN Guangtao, HAO Xiuhua, ZHANG Kai, GAO Xiaoning, LIAO Jie

Frontiers of Medicine 2007, Volume 1, Issue 1,   Pages 87-92 doi: 10.1007/s11684-007-0017-3

Abstract: Reverse transcriptase-polymerase chain reaction was used to detect mRNA expressions of adipose leptin

Keywords： significant difference     intestinal I/R     transcriptase-polymerase     metabolic     central secretory    

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Interaction and independence on methane oxidation of landfill cover soil among three impact factors: water, oxygen and ammonium

Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 2,   Pages 175-185 doi: 10.1007/s11783-011-0320-8

Abstract: To understand the influence patterns and interactions of three important environmental factors, i.e. soil water content, oxygen concentration, and ammonium addition, on methane oxidation, the soils from landfill cover layers were incubated under full factorial parameter settings. In addition to the methane oxidation rate, the quantities and community structures of methanotrophs were analyzed to determine the methane oxidation capacity of the soils. Canonical correspondence analysis was utilized to distinguish the important impact factors. Water content was found to be the most important factor influencing the methane oxidation rate and Type II methanotrophs, and the optimum value was 15% (w/w), which induced methane oxidation rates 10- and 6- times greater than those observed at 5% (w/w) and 20% (w/w), respectively. Ambient oxygen conditions were more suitable for methane oxidation than 3% oxygen. The addition of of ammonium induced different effects on methane oxidation capacity when conducted at low or high water content. With regard to the methanotrophs, Type II was sensitive to the changes of water content, while Type I was influenced by oxygen content. Furthermore, the methanotrophic acidophile, , was detected in soils with a pH of 4.9, which extended their known living environments.

Keywords： quantitative polymerase chain reaction (PCR)     denaturing gradient gel electrophoresis (DGGE)     principal    

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Title Author Date Type Operation

Using RAPD in genotyping and transmission detection

ZHANG Tiejun, ZHOU Xiaoming, ZHANG Tao, YU Shunzhang, JIANG Qingwu, CHEN Yue

Journal Article

Strategy for Carbon Sink and Its Amplification in Marine Fisheries

Tang Qisheng and Liu Hui

Journal Article

Effects of 3-aminobenzamide on poly (ADP-ribose) polymerase expression, apoptosis and cell cycle progression

DU Xiang, ZHAO Hongguang, GUO Wei, GONG Shouliang, WANG Wen

Journal Article

Using pyrosequencing and quantitative PCR to analyze microbial communities

Husen ZHANG

Journal Article

Advances in newly developing therapy for chronic hepatitis C virus infection

Paul J. Pockros

Journal Article

Abnormal expression of c-Myc in human bronchial epithelial cells malignantly transformed by anti-BPDE

FU Juan, JIANG Yiguo, CHEN Xuemin

Journal Article

Smartphone-Imaged HIV-1 Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) on a Chip

Gregory L. Damhorst,Carlos Duarte-Guevara,Weili Chen,Tanmay Ghonge,Brian T. Cunningham,Rashid Bashir

Journal Article

The study of forced vibration for threetower suspension bridge under moving loads

Feng Zhaoxiang,Chen Liang and Miu Changqing

Journal Article

Measurement and Control Technology for the Array of Low Cost PCR Microreactors

Li Xianming ,Zhang Yulin ,Li Jiantian

Journal Article

Experimental Study on the Piezoelectric Prestage Driver of Electrohydraulic Servo Valves

CHENG Guang-ming, SHEN Chuan-liang, YANG Zhi-gang

Journal Article

Expression and clinical implication of PRL-1 and PRL-3 in transitional cell carcinoma of bladder

Bin HAO, Changwei LIU, Huixiang LI

Journal Article

High-level expression of recombinant IgG1 by CHO K1 platform

Ningning Xu, Jianfa Ou, Al-Karim (Al) Gilani, Lufang Zhou, Margaret Liu

Journal Article

Seasonal microbial community shift in a saline sewage treatment plant

Qingmei YAN, Xuxiang ZHANG, Tong ZHANG, Herbert H P FANG

Journal Article

Effect of intestinal ischemia/reperfusion injury on leptin and orexin-A levels

LIN Ji, YAN Guangtao, HAO Xiuhua, ZHANG Kai, GAO Xiaoning, LIAO Jie

Journal Article

Interaction and independence on methane oxidation of landfill cover soil among three impact factors: water, oxygen and ammonium

Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO

Journal Article